Journal: BMC Oral Health
Article Title: Promotion effect of apical tooth germ cell-conditioned medium on osteoblastic differentiation of periodontal ligament stem cells through regulating miR-146a-5p
doi: 10.1186/s12903-022-02485-8
Figure Lengend Snippet: Immunocytochemical staining of PDLSCs. The MACS positive cells showed the expression of STRO-1 ( A ) and CD146 ( C ) by immunocytochemical fluorescent staining, whereas the negative cells didn’t show the expression of STRO-1 ( B ) and CD146 ( D ). PDLSCs could express mesenchymal cell marker vimentin ( E ) but no epithelial cell marker cytokeratin ( F ). Scale bar; 50 μm ( A – D ), 100 μm ( E – G )
Article Snippet: According to the manufacturer’s instructions, single-cell suspensions of PDLCs were incubated with mouse anti-human STRO-1 IgM primary antibody (Life technologies, USA) on a rotator for 1 h at 4 °C, as STRO-1 was the best-known mesenchymal stem cell marker.
Techniques: Staining, Expressing, Marker